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Figure 2 Results of electrophoretic mobility shift assays. Two double-stranded oligonucleotide probes corresponding to the T and C alleles, respectively, of the –1986 T>C polymorphism were labeled with 32P and incubated with nuclear extracts from macrophages (U937 [a] and MonoMac-6 [b]), followed by polyacrylamide gel electrophoresis and autoradiography. The arrow indicates a DNA–protein complex that is more readily detectable with the T probe (lanes 2 and 5) than the C probe (lanes 4 and 9). The DNA–protein complex is readily competed with unlabeled T probe (lanes 6 and 10), weakly competed with unlabeled C probe (lanes 7 and 11), but not affected by a nonspecific competitor (lanes 8 and 12).
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