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Figure 1 Principle of the model. At long pulsing intervals (PIs), complete replenishment of the imaging field (boxes) results in a plateau in contrast intensity (CI) in the frame–CI curve (bottom). After the onset of short PI imaging (from frame no. 0), the microbubble concentration in the imaging field was gradually decreased until a constant level was reached, which can be fit to the decay function. Circles in boxes represent microbubbles; open circles are those to be disrupted during each frame. The arrow in the first box indicates the flow direction, whereas the inverted triangles indicate the advancing fronts of the flow.
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