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Figure 8 Immunohistologic confirmatory analysis demonstrated evidence of transplanted, autologous, "donor" bone marrow cells at 0 (A–D), 14 E–H), and 28 (I–L) days in targeted myocardial tissue demarcated by tissue dye. Primary antibody versus green fluorescence protein (GFP), and secondary antibody conjugated to HRP, DAB chromagen (A, E, I, red arrows) were used to determine cell presence. Negative controls (B, F, J) had nonspecific immunoglobulins used as primary antibody. Hematoxylin–eosin (H&E) stains (C, G, K) show preserved myocardial architecture, and minimal fibrosis is seen on trichrome staining (D, H, L). The animal that received collagen biogel alone did not react nonspecifically to similar antibody staining (M) and had similar preservation of myocardial architecture by H&E (N). Note the collagen biogel fragments (E, closed black arrows) and interstitial biogel deposition (O, blue coloration, trichrome stain). The black (M, N, O) are tissue dye remnants. Positive control for the immunostain was assured using myocardial tissue from a transgenic mouse, positive for GFP (C–D, E–H, K–P, 200x magnification; A–B, I–J, 400x magnification).
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