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Figure 1 (A) Short-term toxicity of nanoparticles of iron oxide (Endorem) towards myogenic precursor cells (MPC) was evaluated immediately after incubation with various Endorem concentrations (0 to 4 mg iron/106 cells) and during different incubation times (4 to 36 h). Cell viability was determined with the WST-1 Roche Diagnostics proliferation kit (Manheim, Germany). The optical density represents mitochondrial enzymatic activity that is proportional to the number of viable cells. There was no evidence of short-term toxicity of Endorem towards MPC. (B) Long-term toxicity of Endorem towards MPC that were incubated with 4 mg iron/106 cells during 24 h. Cell viability was evaluated at 3- to 20-day cultures using the WST-1 kit. Results are expressed as mean optical density ± SD of two experiments performed in triplicate. Compared with control (white bars), there was no evidence of long-term toxicity of Endorem (gray bars) towards MPC in culture. OD = optical density.
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