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Figure 6 Analysis of putative sterol-responsive element (SRE) present in the NOSIII promoter by electrophoretic mobility shift assay (EMSA). (Upper) Representative EMSA was performed with a 32P end-labeled probe containing the SRE element and nuclear extracts from cells treated for 24 h with nLDL (100 µg/ml), glycLDL (100 µg/ml), oxLDL (100 µg/ml), glyc-oxLDL (30 µg/ml), glyc-oxLDL (100 µg/ml), glyc-oxLDL (300 µg/ml), glyc-oxLDL (100 µg/ml, second form), and glyc-oxLDL (100 µg/ml, second form). (Lower) The EMSA was performed with the Oct-1 element used as a control agent to normalize the results (as described in [55]) and with a nuclear extract from cells treated for 24 h with the same lipoproteins. Abbreviations as in Figure 2.
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