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Figure 1 (A) Concentration-dependent binding of (3H)-nisoxetine to membranes obtained from the right atria (RA) of 5 children and 2 young adults in group A (5 males and 2 females; mean age 14 ± 3 years) and 11 elderly patients in group B (8 males and 3 females; mean age 60 ± 3 years) (New York Heart Association functional class 1.73 ± 0.19). Ordinate: (3H)-nisoxetine concentration in fmol specifically bound/mg protein; abscissa: (3H)-nisoxetine concentration in nmol/l. Data are presented as the mean value ± SEM. ***p < 0.001 vs. group A. (B) Inhibition of specific (3H)-nisoxetine binding (2 nmol/l) to RA membranes obtained from 8 elderly patients (6 males and 2 females; mean age 67 ± 3 years) by uptake inhibitors. Ordinate: specific (3H)-nisoxetine binding as percent; abscissa: molar concentrations of uptake inhibitors. Data are presented as the mean value ± SEM of two experiments performed in triplicate with log (mol/l) IC50 values: desipramine –7.51 ± 0.20; nisoxetine –7.09 ± 0.06; cocaine –5.52 ± 0.08; and GBR 12909 –5.35 ± 0.10.
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