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Figure 2 In vitro induction of tolerance to heat shock protein (HSP) 65 by co-culture of tolerized splenocytes with HSP65 responder lymph node cells. To assess in vitro tolerization, 105 splenocytes from HSP65 or bovine serum albumin (BSA)-fed (i.e., mice that were fed 5 times with HSP65, 50 µg/dose or BSA) were incubated in the presence of 104 lymph node cells from Mycobacterium tuberculosis immunized mice in the presence of different concentrations of HSP65. The figure represents average values obtained from three studies, each repeated twice. *p < 0.05. Hatched bar = BSA-fed splenocytes; solid bar = HSP-fed splenocytes.
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