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Figure 1 Oral feeding with heat shock protein (HSP) 65 tolerizes immune responses to HSP65 in vivo. Low-density lipoprotein receptor deficient mice were immunized with HSP65 (10 µg/mouse) emulsified in incomplete Freunds adjuvant in the hind footpad. The mice were fed with either HSP65 or bovine serum albumin (BSA) before immunization as described in the Methods section. Lymphocytes obtained from draining inguinal lymph node cells of mice from both groups were cultured in the presence of different concentrations of HSP65 (A). Reactivity to HSP65 was determined by thymidine incorporation by lymphocytes and expressed as stimulation index. (B) Reactivity to HSP65 was evaluated in a similar manner in HSP65- or BSA-fed mice immunized with heat-killed preparation of Mycobacterium tuberculosis. *p < 0.01. Hatched bar = BSA-fed; solid bar = HSP65-fed.
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