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Figure 3 Paraffin sections of case 1 using alkaline phosphatase-conjugated antibodies directed against (staining methods as outlined in Methods section): (A) human immunoglobulin (Ig)G demonstrating diffuse deposition of IgG; (B) human IgM demonstrating focal areas of deposition; (C) negative terminal deoxynucleoleotidyl transferase-mediated dUTP-biotin nick end-labeling staining for apoptosis; (D) negative staining for pan B cell marker (CD 20); (E) focal areas of pan T cell marker (CD 43); and (F) focal area of Granzyme B positive staining (magnification 25 x 10 x 1.25).
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