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Figure 7 Immunohistochemical analysis of extracellular matrix (ECM) of cell-depleted areas in human coronary stent neointimal tissue sections adjacent to the section of Figure 2C. In modified Movat staining, two kinds of cell-depleted areas were characterized. The collagen-rich ECM (pinkish yellow, thick arrow) is colocalized with the dense matrix seen in Figure 2C, and the proteoglycan-rich ECM (blue, thin arrow) with the loose matrix. Fibrin and/or fibrinogen are labeled as red (asterisk). Versican is abundant in the proteoglycan-rich area (brown, thin arrow), but is spared in the collagen-rich ECM (thick arrow). The distribution of hyaluronan is similar to that of versican. The biglycan-stained area is mostly colocalized with the dense collagen-rich matrix (brown, thick arrow). A few fibrin-labeled areas (dark brown) assessed by T2G1 antibodies are shown, counterstaining with methylgreen. Bar = 100 µm.
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