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Figure 2 Results of measurement of tissue inhibitor of matrix metalloproteinase (TIMP) protein (A) and messenger ribonucleic acid (mRNA) (B) in left ventricular extracts of Dahl salt-sensitive (DS) and Dahl salt-resistant (DR) rats at the left ventricular hypertrophy (LVH) and congestive heart failure (CHF) stages. The TIMP-2 and -4 proteins and mRNA were measured by Western and Northern blot analyses, respectively. The TIMP mRNA level was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA. The data are expressed as arbitrary units (values for 11-week-old DR rats were set at 1.0, and remaining values were adjusted accordingly) and mean values ± SEM. *p < 0.01. p < 0.05. (A) p_a = 0.0207, p_t = 0.7292 and p_i = 0.0127 for TIMP-2 by Western blot; p_a = 0.0002, p_t = 0.0002 and p_i = 0.0021 for TIMP-4 by Western blot. (B) p_a = 0.0746, p_t < 0.0001 and p_i = 0.1669 for TIMP-2 mRNA by Northern blot; p_a = 0.0007, p_t = 0.0050 and p_i = 0.1804 for TIMP-4 mRNA by Northern blot. W = weeks.

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