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Figure 2 Effect of N-2-mercaptopropionyl glycine (MPG) on atrial natriuretic factor (ANF) mRNA expression. Total RNA was isolated from hearts treated with (+) or without (–) MPG and analyzed for expression of ANF mRNA by northern blot. A representative northern blot analysis result is shown in the upper panel. Sham = sham-operated mice; TAC = TAC-operated mice. Lower panel shows the quantitative analysis of ANF mRNA expression relative to ß-actin mRNA based on densitometric measurements on northern blot. Data are expressed as mean ± SEM (n = 3). Two-way analysis for variance (ANOVA) was used to test significance, with one factor being sham versus TAC operation and the other factor being vehicle versus MPG treatment. As we faced a significant interaction, we have analyzed the data among groups by one-way ANOVA and Tukey-Kramer’s post hoc test.
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