Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 2 (a) The effects of pretreatment (1 h) with activator protein-1 (AP-1) consensus (AP-1 cons) or AP-1 mutant (AP-1 mut) decoy oligodeoxynucleotide (dODN) (10 µmol/l each) on phorbol dibutyrate (1 µmol/l)-induced nuclear translocation of AP-1 in endothelium-intact segments of the porcine left anterior descending coronary artery from the same animal. The segments were incubated in Waymouth medium containing 10% fetal bovine serum for 1 h at 37°C, followed by electrophoretic mobility shift analysis. The results shown are representative of at least two further experiments with segments from different animals. (b) In vivo treatment of porcine coronary arteries from the same animal, with AP-1 consensus dODN resulting in a diminished nuclear translocation of AP-1 relative to the buffer control 48 h after percutaneous transluminal coronary angioplasty (upper box), although nuclear translocation of CCAAT/enhancer binding protein (C/EBP)ß and - was not affected. The results shown are representative of those obtained with four different animals. Identification of the C/EBP family members was done by supershift analysis (10).
|
