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Figure 3 (A) Effects of ET-1 and PDGF on 3H-TdR incorporation into coronary artery SMCs in vitro. After 96- or 72-h serum starvation, quiescent SMCs were either incubated with ET-1, PDGF-AA, PDGF-BB or PDGF-AB for 48 h, or prestimulated with ET-1 for 24 h; thereafter, PDGF-AA, -AB or -BB was added to cultures for 48 h, respectively. Incorporation of 3H-TdR (1 µCi/ml) was measured during last 24 h. Six parallel wells were used for the determinations, and the experiment was repeated twice. (B) Effects of ET-1 and PDGF into coronary artery SMC migration in vitro. The cells were seeded in porous membranes separating the upper and lower chambers. After 2 h, ET-1, PDGF-AA, PDGF-BB and PDGF-AB were added to the lower chamber and incubated for 24 h at 37°C. Migrated cells on the lower side of the filter were quantitated by counting the cells on the lower side of the filter. Three parallel wells were used for the determinations, and the experiment was repeated twice. Data are expressed as the mean value ± SEM. NIL = no treatment.
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