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Figure 1 Effect of Bosentan (Ro 47-0203), an ET-1 receptor antagonist, on (A) incidence and (B) mean score of intimal thickness in epicardial arteries and intramyocardial arterioles of rat cardiac allografts. Allograft recipients were given Bosentan, 100 mg/kg per day (n = 9), or vehicle (n = 7), and, as background immunosuppression, CsA, 2 mg/kg per day for the first week and 1 mg/kg per day thereafter. The grafts were removed 60 days after transplantation. Data are given as the mean value ± SEM; data were analyzed by using the Mann-Whitney U test. Grade 0 = normal artery with intact internal elastic lamina; grade 1 = <10% occlusion of the lumen by arterial intimal thickening and proliferation, disruption of the internal elastic lamina and the presence of some foam or vacuolated ECs; grade 2 = >10% but <50% occlusion of the lumen; grade 3 = >50% but <100% occlusion of the lumen; and grade 4 = 100% vessel occlusion of the lumen. Photomicrographs of the coronary arteries of vehicle-treated (C) and Ro 47-0203–treated (D) rat cardiac allografts. Original magnification x200. Hematoxylin-eosin and resorcin-fuchsin staining for internal elastic lamina.
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