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Figure 2 Equal amounts of immunoprecipitated myocardial proteins were loaded onto 5% to 20% sodium dodecyl sulfate–polyacrylamide gradient gels and visualized by silver staining. Immunoprecipitation was performed with sepharose-coupled antibodies collected during the first regeneration cycle of session 1 of IA from the CE of three patients with DCM and with sepharose-coupled antibodies obtained from the combined CE of two healthy blood donors. Immunoprecipitation with myocardial membrane-associated protein fraction (A) or with myocardial membrane protein fraction (B). M = molecular weight marker; AG = antigen (myocardial protein fraction) alone, no antibodies added; P1–3 = sepharose-coupled antibodies obtained from the CE of a patient with DCM plus myocardial protein fraction; C = sepharose-coupled antibodies obtained from the CE of control subjects (combined CE of two healthy male blood donors) plus myocardial protein fraction; AB = sepharose-coupled antibodies alone.
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