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Figure 3 The effect of nitroglycerin (NTG) on matrix metalloproteinase (MMP)-9 messenger ribonucleic acid (mRNA) levels in human monocyte-derived macrophages (MDMs). Cells were exposed to control (CTRL) media (a), NTG 200 pmol (b) or NTG 2,000 pmol (c) for 4 h. Matrix metalloproteinase-9 mRNA levels were measured by competitive reverse transcription-polymerase chain reaction (described in Methods). Competitor mRNA concentrations were 8.56 (Lane 1), 17.13 (Lane 2), 34.25 (Lane 3), 68.5 (Lane 4) and 137 (Lane 5) attomoles. Arrows represent MMP-9 target (lower band) versus competitor (upper band) equivalence points for each condition. (d) Quantification of RT-PCR bands was determined by gel densitometry. Results represent the mean ± SEM for analysis of four independent experiments and do not represent the analysis in (a to c) alone. **p < 0.005 compared with control cells. ***p < 0.001 compared with control cells.
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