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Figure 2 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) zymography of media conditioned by human monocyte-derived macrophages exposed to nitroglycerin (NTG) for 4 h. (a) Cells were exposed for 4 h to control media (Lanes 1 and 2), glycerol (GLY) 200 pmol (Lanes 3 and 4), GLY 2,000 pmol (Lanes 5 and 6), NTG 200 pmol (Lanes 7 and 8), NTG 2,000 pmol (Lanes 9 and 10), 3-morpholinosydnoimine hydrochloride 200 pmol (Lanes 11 and 12) or S-nitroso-N-acetylopenicillamine 200 pmol (Lanes 13 and 14). Aliquots of conditioned media, normalized to 4.5 µg total protein, were run on SDS-PAGE (10%[w/v]gel) supplemented with 1 mg/ml gelatin substrate under nonreducing conditions. Zones of clearing indicate gelatinolytic activity of latent matrix metalloproteinase (MMP)-9. (b) Scanning analysis of MMP-9 secreted by human MDMs. Results represent the mean ± SEM for analysis of four independent experiments and do not represent the analysis in (a) alone. *p < 0.05 compared with control cells. **p < 0.005 compared with control cells. ***p < 0.0001 compared to control cells.
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