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Figure 3 Production of transforming growth factor-beta1 (TGF-beta1) in the monocytes of hypercholesterolemic patients (n = 9) at baseline and after four to six weeks of diet plus pravastatin (40 mg/day) treatment. A conditioned medium of unstimulated cultures was collected after 24 h, and the biologic activity of TGF-beta1 was evaluated after acidification for mink-lung epithelial cell bioassay. Mink-lung cells respond to TGF-beta1 released in the culture medium, with a decrease in deoxyribonucleic acid synthesis, as evaluated by 3H-thymidine incorporation. A comparison with the standard curve and multiplication by the dilution factor (1:10) yielded TGF-beta1 concentration (ng/ml) in the conditioned medium of monocyte cultures.
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