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Figure 4 (A) Representative Western blot of bcl2 and bax protein expression in the cardiac myocyte and cardiac fibroblast after exposure to different dosages of hydrogen peroxide (H2O2) (300, 500, 800 µM) for 30 min. The experiment was repeated at least three times, with n = 3, for both cardiac myocytes and fibroblasts with similar results. C = control cells, not exposed to oxidative stress and assayed after 30 min. Coomassie blue staining was used to confirm equal loading. (B) Densitometric analysis of bcl2 and bax protein in the neonatal cardiomyocytes and fibroblasts (n = 3 each dose; two-way analysis of variance, p < 0.01). Results are expressed as mean ± SD. t test was used for individual comparisons. *p < 0.05, myocyte versus fibroblast for differences in bax protein expression (bax-M vs. bax-F). M = myocyte, F = fibroblast.
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