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Figure 2 Histology and immunohistology of representative examples of coronary atherectomy specimens. (a, c, e, g, i) Typical example of a R– specimen. (a) A modified trichrome stain with erythrocytes staining yellow; fibrin stains red, collagen stains green and elastic fibers stain dark blue. The internal elastic lamina was utilized as the landmark for identifying the intimal-medial interface. (c) A parallel section where red nuclear staining represents a positive TUNEL reaction. (e) Immunoreactivity with anti-proliferating cell nuclear antigen (PCNA). Note few positive nuclei. (b, d, f, h, j) Typical example of a R+ specimen. (b) A modified trichrome stain. Note that in this case the internal elastic lamina is present, because directional coronary atherectomy cut has also removed deeper parts of the arterial wall. Parts of the medial layer are shown at the bottom of the photomicrograph. (d) A parallel section where red nuclear immunoreactivity represents positive TUNEL reaction. (f) Anti-PCNA immunoreactivity. The areas highlighted by rectangles are shown in g, h, i and j at a higher magnification. The scale bars represent 10 µm.
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