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Figure 3 Immunohistochemical detection of heat shock protein (HSP)60/65. Immunoglobulin G (IgG) was purified from the sera of mice immunized with HSP65. Purified IgG was cross-reactive with HSP60 employing enzyme-linked immunosorbent assay. The IgG from HSP65 immunized mice was used to immunostain aortic sinus sections from low density lipoprotein receptor-deficient mice with the Histomouse kit. (A) Positive staining of the endothelial cells; (B) a similar section stained with control mouse IgG.
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