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Figure 7 Immunohistochemical staining for recombinant human tissue factor pathway inhibitor (rTFPI) in arterial sections obtained from carotid arteries transfected with retro-TFPI under uninduced conditions (i.e., no infusion of doxycycline) (A) and after administration of doxycycline to an animal subjected to a previous injury (B) or after administration of doxycycline to an animal not subjected to a previous arterial injury (C). After doxycycline infusion, a diffuse and intense reactivity was present across the arterial wall, although no staining was present under uninduced conditions. No significant differences in rTFPI expression were observed in the arterial wall between the two groups of animals. Recombinant TFPI was probed with a monoclonal antibody against a 6-histidine tag present only in the recombinant protein, thus allowing detection of rTFPI from the wild-type protein. (D) Histologic section of an artery obtained during doxycycline infusion and probed with an unrelated mouse monoclonal antibody (Anti-Xpress, Invitrogen). No cross reactivity is present in this section (see Methods for further details).
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