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Figure 3 Ex vivo secretion of recombinant tissue factor pathway inhibitor (TFPI) by rabbit smooth muscle cells (SMCs). The rTFPI levels were measured by a two-antibody sandwich assay, using a monoclonal antibody against a 6-histidine tag present only in the recombinant protein, thus excluding the contribution of the wild-type TFPI. A progressive increase in rTFPI levels was observed, peaking at 12 to 24 h after doxycycline induction.
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