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Figure 3 GIRK1 and GIRK4 are expressed in both the atrium and ventricle of the mouse. Total ribonucleic acid (RNA) from the atrium and ventricle of the adult mouse was amplified by reverse transcription-polymerase chain reaction (PCR) using primers specific for GIRK1, GIRK4 or myosin light chain (MLC)-2a. RNA from NIH-3T3 cells was used as a negative control agent, and complementary deoxyribonucleic acid (cDNA) for mouse GIRK1, GIRK4 and MLC-2a was used as a positive control agent in PCR.
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