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Figure 2 Representative and summarized myocardial tubulin data for normal patients and patients with AS. (Top) Immunoblot prepared from left ventricular biopsies from one patient from each of the four study groups defined in Figure 1. For comparison of the ratio of free heterodimer (lanes #1) to polymerized microtubule (lanes #2) tubulin in the four biopsies, an equal amount of tubulin protein was loaded for each of the free tubulin lanes, and an equal volume of the polymerized tubulin (microtubule) fraction was loaded for each of the polymerized tubulin lanes. (Bottom) Summary semiquantitative tubulin data for all of the patients in the four groups defined in Figure 1; the values were obtained from immunoblots wherein known quantities of concurrently run pure beta-tubulin were used as a standard, and the integrated optical density of the standards was compared with that of the unknown samples. Statistical analysis by one factor analysis of variance, which showed a significant (p < 0.05) F-ratio, was followed by a Fisher protected least significant difference post-hoc test. *p < 0.05 for difference from the value for the Control group. AS = aortic stenosis.
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