Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 8 A, Representative Western blot analysis showing eNOS protein expression in neutrophils obtained from a patient with AMI and from a control subject. (Densitometric analysis in arbitrary units—control subject: 100; patient with AMI: 16 ± 4 [n = 8]; *p < 0.05.) B, Representative gel mobility shift assay to analyze the presence of the cytosolic proteins that bind to 3'-UTR of eNOS mRNA in neutrophils from both a control subject and a representative patient with AMI. Competitive experiments were performed with 1,000 ng of unlabeled UTR-UC demonstrating the specificity of the binding. Treatment of the neutrophil cytosolic extracts from patients with AMI with proteinase K (Prot K, 87 µg/ml) before incubation with the UTR-L RNA fully abolished the gel-shifted band. 3'-UTR = 3'-untranslated region; AMI = acute myocardial infarction.
|
