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Figure 6 Representative gel mobility shift assay of six different experiments to test the effect of simvastatin on the binding activity of human neutrophil cytosolic proteins to the 3'-UTR of eNOS mRNA. The neutrophils were stimulated with TNF-alpha (500 U/ml) for 8 h in the presence and absence of increasing doses of simvastatin. The cytosolic extracts were analyzed using the labeled UTR-UC probe. (Densitometric analysis in arbitrary units—basal: 100; TNF-alpha: 180 ± 10; TNF-alpha + simvastatin 10–7 mol/liter: 142 ± 17*; TNF-alpha + simvastatin 10–6 mol/liter: 120 ± 9*; TNF-alpha + simvastatin 10–5 mol/liter: 45 ± 7*; [n = 6];*p < 0.05 vs. TNF-alpha–incubated neutrophils.) Abbreviations as in Figure 1.
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