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Figure 5 A, Representative gel mobility shift assay of six different experiments to analyze the regulation of neutrophil cytosolic protein binding activity by TNF-alpha. Human neutrophils were incubated with TNF-alpha (500 U/ml) for different periods, and their cytosolic extracts were analyzed by gel mobility shift assay using the labeled UTR-UC probe. B, Competitive experiments with greater amounts of unlabeled UTR-UC probe, demonstrating the specificity of the binding. For the competition experiments, human neutrophils were incubated with TNF-alpha (500 U/ml) for 8 h. The amount of radiolabeled RNA used was 1 ng. 1x = 10 ng of RNA.
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