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Figure 4 A, Representative gel mobility shift assay of six different experiments with the 3'-UTR of eNOS mRNA. The autoradiograph shows the results of gel mobility shift assays using the labeled UTR-L probe incubated with neutrophil cytosolic extracts (lane 1). Competitive studies were performed with 1,000 ng of unlabeled UTR-L (lane 2). Two RNA probes containing specific sequences within UTR-L were also tested as competitors: 1,000 ng of UTR-UC (lane 3) and 1,000 ng of UTR-AU (lane 4). The amount of the labeled UTR-L used was 1 ng. B, Treatment of neutrophil cytosolic extracts with proteinase K (87 µg/ml) before incubation with UTR-L RNA fully abolished the gel-shifted band. Abbreviations as in Figure 1.
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