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Figure 4 Effect of angiotensin II and PDGF-BB on TnC mRNA and protein expression. Quiescent culture of porcine adventitial cells were treated with 1 nM PDGF-BB or 100 µM angiotensin II for the indicated times (in hours). Total RNA was extracted and analyzed by Northern blot and hybridized with TnC. To control for loading, the blot was rehybridized with the GAPDH cDNA probe. (B) For Western blot analysis, the media from cultured cells treated with angiotensin II or PDGF-BB for 8 and 24 h were collected and analyzed using 1:5,000 dilution of chicken antibodies to the recombinant fibrinogen-like domain of TnC. GAPDH = glyceraldehyde-phosphate dehydrogenase; TnC = tenascin-C.
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