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Figure 2 Effect of TNF-alpha depletion on myocardial TNF-alpha mRNA expression in the ischemic rat heart. A, Representative PCR analysis of RNA samples (in duplicate). B, Relative optical densities of TNF-alpha PCR signal. Data were normalized to relative GAPD PCR signal. 90' Perf = 90 min of normal nonischemic perfusion, serving as control group (n = 4); 60' Isch = hearts undergoing 1 h of global cardioplegic ischemia (n = 5); Anti TNF = hearts receiving monoclonal anti–TNF-alpha antibodies in the cardioplegia (n = 5) just before ischemia, showing significantly lower TNF-alpha mRNA expression than detected in untreated ischemic hearts (*p < 0.03).
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