Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 1 (a) Part of the nucleotide sequence of the human angiotensin II type 2-receptor gene. The position of the G>A substitution at position +1675 is marked with an arrow. First row: DNA-sequence of a patient carrying the G-allele. Second row: DNA-sequence of a patient carrying both alleles (G/A). Third row: DNA-sequence of a patient carrying the A-allele. (b) Representative example of a SSCP-gel showing the characteristic band-pattern of the +1675 G/A polymorphism after denaturing and loading on a 10% PAA gel at 4°C. Lane 1: PCR-product of a heterozygote patient designated G/A. Lane 2: PCR-product of a patient carrying the G-allele (G). Lane 3: PCR-product of a patient carrying the A-allele (A). Lane 4 and 5: PCR-products of patients carrying the G-allele.
|
