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Figure 6 The electrophoretic mobility shift assay showing the effect of ADMA, SDMA and LDL lipoproteins on the activation of NF-B. Nuclear extracts were isolated from ECV 304 human endothelial cells after incubation with the various substances for 24 h. Binding reactions were carried out by mixing nuclear proteins with a ³²P-labeled double-stranded DNA oligonucleotide corresponding to the published NF-B-binding domain. In some experiments, unlabeled oligonucleotide corresponding to the same NF-B-binding domain ("competitor") or to the SP-1 binding domain ("noncomp") was utilized. This experiment was replicated 3 times. ADMA = asymmetric dimethylarginine; LDL = low-density lipoprotein; MCP-1 = monocyte chemotactic protein; nLDL = native low-density lipoprotein; oxLDL = oxidized low-density lipoprotein; SDMA = symmetric dimethylarginine.

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