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Figure 2 Complete inhibition by ET-1 of Iso-induced apoptosis. Cultured cardiac myocytes were treated for 48 h in serum-free media in the presence or absence of Iso (10–5 mol/liter) and the indicated concentrations of ET-1. TUNEL-positive nuclei were counted and are expressed as the percentage of total nuclei. An average of 400 to 500 nuclei were counted from random fields in each slide. Results are the mean ± SE of three independent experiments.
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