Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 2 Decrease in ligand-binding to human beta1-AR expressed in Sf9 cells (a) in the presence of constant 3 nmol/liter [3H]CGP 12177 and the indicated concentrations of anti-beta1-AR, or (b) in the presence of a fixed concentration of the different anti-beta1-AR (mouse, 7 nmol/liter; rabbit, 70 nmol/liter; human: 2 µmol/liter) and various concentrations of [3H]CGP 12177 (3 x 10–11 to 5 x 10–9 mol/liter). Error bars indicate the mean ± SD of three experiments. Bmax = total ligand binding capacity; KD = receptor-ligand dissociation constant.
|
