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Figure 1 Detection of anti-beta1-AR by various approaches. (a) Western blots of crude cell lysates, and (b) immunoprecipitates of membrane-biotinylated Sf9 cells expressing recombinant human beta-1-AR (R) or the wildtype vector (C), and (c) fixed and permeabilized, or (d) intact unfixed Sf9-beta1 cells were probed with either (a, c, d) domain-specific rabbit anti-beta1-N/-ECII/-C (Rabbit) or mouse anti-beta1-ECII (Mouse), or (b) with POD-coupled streptavidin (see Methods). Their respective immunoreactivities are compared with those of anti-beta1-ECII-positive or -negative cardiomyopathic patients (Human, Pat. ß1-Pos./-Neg.). Note, that SDS-denatured beta1-AR (lane a) are stained less clearly (Rabbit) or not at all (Mouse, Human) by anti-beta1-ECII antibodies, whereas all of them immunoprecipitate (lane b) or recognize the native beta1-AR (lane d).
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