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Figure 1 (Top panel) Fluorescent labeling of beta-adrenoreceptors (green) and nuclei (blue with 4'6'-diamidino-2-phenylindole chloride, 0.1 g/ml). (A) Normal segment (B) Dysfunctional segment. (Middle panel) Mallory’s trichrome stain for interstitial fibrosis. (A) Normal segment; (B) Dysfunctional segment. (Lower panel) Tissue Doppler velocities. Am = late diastolic myocardial velocity; Em = early diastolic myocardial velocity; Sm = systolic myocardial velocity; from normal (A) and dysfunctional (B) segments. Notice the higher beta-adrenergic receptor density, the lower amount of interstitial fibrosis and the preserved Sm and Em velocities in the normal segment.
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