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Figure 5 Cell death–inducing activities exist in the Centricon C-100 retentates. Pericardial fluid was fractionated with Centricon C-100 as described under "Methods." A) A representative photograph showing a retentate (R) and a filtrate (F) electrophoresced by an SDS-polyacrylamide (2% to 15%) gradient gel and stained with Coomassie Brilliant Blue. MW = molecular weight. B) In each retentate and filtrate, F2 cell survival assays were performed as described in the legend for Figure 1. In each experiment, the viability of the well incubated with 10% FBS was set to 100%. All of the experiments were performed in triplicate for each patient sample and repeated at least three times. Results are the mean ± SD of each patient group.
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