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Figure 1 Pericardial fluid from patients with UA induces loss of F2 cells. F2 cells were plated in 96 well plates and maintained for 48 h in the presence of 10% pericardial fluid obtained during coronary artery bypass surgery. Cell viability was assayed using 3-(4,5-dimethyl thiaziazol-2-yl)-2,5-diphenyl tetrazolium bromide as described under "Methods." In each experiment, the viability of the well incubated with 10% FBS was set to 100%. All of the experiments were performed in triplicate for each patient sample and repeated at least three times. Results are the mean ± SD of each patient group.
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