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Figure 4 Immunohistochemistry showed marked increase in endothelial cell and smooth muscle cell count (not associated with a vascular lumen) in groups treated with US during administration of VEGF compared with groups treated with VEGF alone. A) shows endothelial cells as shown by vWF staining in a rat heart from an animal treated with VEGF alone and B) shows endothelial cells in a rat heart treated with VEGF + US. C) shows smooth muscle cells in a rat heart from an animal treated with VEGF alone and D) shows a rat heart from an animal treated with VEGF + US. Positively stained cells were counted at 200x.
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