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Figure 5 Electrophoretic mobility shift assays demonstrating the effects of NO donor, spermine/NO, on NF-kappaB DNA binding activity (representative of three similar experiments). Supershifted immunoreactive band using antibodies against the p50 and p65 subunits of NF-kappaB are indicated by the arrows. NF-kappaB = nuclear factor kappa B; NO = nitric oxide; Spermine = the carrier vehicle for the NO donor.
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