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Figure 2 A, Immunohistochemical staining (alpha-actin). Neointima 101 days after stenting (subject no. 324/97). Compact layer of alpha-actin testing positive cells (red) in the luminal zone of the intima. Loose infiltration with alpha-actin–positive cells of the intercellular matrix in the thicker, outer layer of the neointima. (Magnification x45, reduced by 65%.) B, Light microscopic specimen. Stent neointima after removal of the stent struts (s) (subject no. 174/97). Directly adjacent to the alloplastic stent filaments no alpha-actin–positive SMCs (red) can be detected. (Magnification x45, reduced by 65%.) C, Immunohistochemical staining after removal of stent struts (subject no. 38/95). Factor 8-negative cells form the borderline between the lumen (l) and neointima (n) 68 days after stenting. Endothelial cells of the hyperemic vasa vasorum (v) are tested factor 8–positive. Indentation of stent struts. (Magnification x35, reduced by 65%.) D, Immunohistochemical staining (factor 8-positive = red). Complete endothelialization of the neointima by mature endothelial cells 101 days after stenting (subject no. 324/97). Upper, lumen; lower, neointima (distal stent). (Magnification x75, reduced by 65%.)
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