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Figure 5 Effect of 5HT2 receptor antagonist (LY281067) on 5HT induced proliferation of LDL or mox-LDL pretreated VSMC. Synchronized VSMCs were stimulated with different concentrations of LDL or mox-LDL. 10 µg/mL of LY281067 was added 4 h before the addition of 5HT. After 24 h of incubation with LDL or mox-LDL, 50 µmol/L of 5HT was added and incubated for an additional 24 h. 3H-thymidine (1 µCi/plate) was added during the last 5 h of incubation. One hundred percent equals the baseline value for 3H-thymidine incorporation into DNA (276 ± 17 cpm). Values shown are mean ± standard error. Abbreviations as in Table 2. n = 24; *p < 0.05 compared with LDL group control; **p < 0.05 compared with corresponding LDL and 5HT group; @p < 0.01 compared with mox-LDL group control; &p < 0.001 compared with 5HT; $p < 0.001 compared with corresponding mox-LDL and 5HT group.
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