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Figure 4 Effect of LDL or mox-LDL and 5HT on cell number. Vascular smooth muscle cells (5 x 104) were plated in 2 mL of serum free medium. After synchronization, vascular smooth muscle cells were stimulated with different concentrations of LDL or mox-LDL for 24 h, followed by incubation with 50 µmol/L of 5HT for 24 h. Then cells were trypsinized and counted as described in the Materials and Methods section. Data represent the mean ± SE from triplicate dishes from three different experiments. Baseline value is 256, 650 ± 13, 430 cells/plate. Abbreviations as in Figure 2. n = 12. *p < 0.01 compared with control; **p < 0.05 compared with corresponding control.
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