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Figure 8 Expression of CASP-3, BCL-2 and BAX protein. (A) Whereas p32 isoform of CASP-3 was detected in all patients, the p17 isoform was detected only in patients with AF (lanes 2 and 5) and in some patients with LLVEF (lanes 3 and 4). (B) A lower expression of BCL-2 was observed in patients with AF (lanes 2 and 5) and with LLVEF (lanes 3, 4 and 7) compared with control patients (lanes 1 and 6) while BAX expression was unchanged. In all samples analyzed, a 23-kDa band was detected with antibody directed against BCL-2, the expression of which was enhanced in patients with AF (lanes 2 and 5) or with LLVEF (lanes 3, 4 and 7) compared with control patients (lanes 1 and 6). Tubulin was used as a control of equal loading and, except for patient in lane 2, soluble tubulin was the predominant form.
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