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Figure 3 cAMP-levels of CHW-beta-1 cells upon incubation with different patient IgG preparations. The cells were stimulated with 10 µmol/liter (–) isoproterenol (hatched bars) or not (black bars) either (a: left) (error bars: ± SD) in the absence (Controls) or presence of IgG fractions from antibody-negative control subjects (Healthy, negatives), or (b: right) (error bars: ± SD of three independent experiments) after preincubation with immunofluorescence-positive IgG fractions from an HHD patient also staining Sf9 control cells (left), a VHD patient with concomitant ICM (middle) and the only positive control subject (right). Significant differences compared with antibody-negative healthy individuals are denoted by (*)p < 0.1, *p < 0.05 and **p < 0.001 (Scheffé’s F-test, post-hoc multiple comparison).
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