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Figure 2 Detection of anti-beta-AR antibodies by immunofluorescence microscopy. Intact native Sf9 cells expressing recombinant human beta-AR (ß1/ß2), or infected with wild-type vector (W) were probed with subtype-specific anti-beta-AR rabbit antibodies (5) (Rabbit, anti-ß1/ß2), with IgG from an antibody-negative healthy subject (Healthy), or with IgG from a patient with chronic valvular and concomitant ischemic heart disease recognizing only the beta-1-AR (VHD), or a patient with hypertensive heart disease and concomitant collagenosis recognizing both beta-AR bearing Sf9 cells and wild-type virus-infected control cells (nonspecific staining, HHD).
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