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Figure 1 Representative figures of the polymerase chain reaction products after agarose gel electrophoresis. Coamplification of each of the calcium-handling protein genes with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) showed no interference between the primer pairs, and the two deoxyribonucleic acid bands could be well separated by gel electrophoresis (upper band for calcium-handling protein and lower band for GAPDH). The upper panel shows the polymerase chain reaction results from Patient No. 13 and the lower panel from Patient No. 21, and the samples for each calcium-handling gene were right atrial appendage, right atrial free wall, left atrial appendage and left atrial free wall from left to right. Despite a large variation of messenger ribonucleic acid (mRNA) levels of L-type calcium channel and calcium adenosine triphosphatase (Ca2+-ATPase) between patients, the mRNA levels of the genes among the four atrial sampling sites were not significantly different in the same patient. Lane specification: lanes 1 and 22 = molecular weight marker; lanes 2 to 5 = L-type calcium channel; lanes 6 to 9 = Ca2+-ATPase; lanes 10 to 13 = calsequestrin (CQ); lanes 14 to 17 = ryanodine receptor (RYR); lanes 18 to 21 = phospholamban (PLB).
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