Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 2 Neutrophil CD11b expression. Peripheral blood samples obtained from healthy adult volunteers, incubated with 3.5 µg/ml chimeric 7E3 Fab at 37°C for 30 min, showed a decrease in each individual’s neutrophil CD11b expression (paired t test, n = 5). Peripheral whole blood samples obtained from healthy adult volunteers were incubated with 3.5 µg/ml chimeric 7E3 Fab at 37°C for 30 min and then stimulated with fMLP (10 nmol/liter) to determine if this apparent decrease in detectable CD11b was of functional significance. After stimulation, neutrophil CD11b expression increased dramatically regardless of the presence of chimeric 7E3 Fab (paired t test, n = 5). Two phycoerythrin-conjugated monoclonal antibodies were studied to confirm this finding (Leu-15, Becton Dickinson Immunocytometry Systems, San Jose, California; 2LPM19c, DAKOpatts, Denmark).
|
